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hcmv pp65 peptide pool  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec hcmv pp65 peptide pool
    Hcmv Pp65 Peptide Pool, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcmv+pp65+peptide+pool/pp65/pm38923358-112-25-31
    Average 90 stars, based on 1 article reviews
    hcmv pp65 peptide pool - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Engineered HCMV-infected APCs enable the identification of new immunodominant HLA-restricted epitopes of anti-HCMV T-cell immunity.
    Article Snippet: Funding information German Research Foundation, Grant/Award Number: 431451204; German Centre for Infection Research, Grant/Award Numbers: TTU07.838_00, TTU07.838_01, TTU07.815.00 Complications due to HCMV infection or reactivation remain a challenging clinical problem in immunocompromised patients, mainly due to insufficient or absent T-cell functionality.. Knowledge of viral targets is crucial to improve monitoring of high-risk patients and optimise antiviral T-cell therapy.. To expand the epitope spectrum, genetically-engineered dendritic cells (DCs) and fibroblasts were designed to secrete soluble (s)HLA-A*11:01 and infected with an HCMV mutant lacking immune evasion molecules (US2-6 + 11).

    Concentration Assay:

    Article Title: Engineered HCMV-infected APCs enable the identification of new immunodominant HLA-restricted epitopes of anti-HCMV T-cell immunity.
    Article Snippet: Funding information German Research Foundation, Grant/Award Number: 431451204; German Centre for Infection Research, Grant/Award Numbers: TTU07.838_00, TTU07.838_01, TTU07.815.00 Complications due to HCMV infection or reactivation remain a challenging clinical problem in immunocompromised patients, mainly due to insufficient or absent T-cell functionality.. Knowledge of viral targets is crucial to improve monitoring of high-risk patients and optimise antiviral T-cell therapy.. To expand the epitope spectrum, genetically-engineered dendritic cells (DCs) and fibroblasts were designed to secrete soluble (s)HLA-A*11:01 and infected with an HCMV mutant lacking immune evasion molecules (US2-6 + 11).



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    Miltenyi Biotec gmp grade cmvpp65 peptide pool
    Comparison of ex vivo restimulation of CMV-specific T-cells by antigenic restimulation by platform . Restimulation frequencies of CMV-specific T-cells were compared between all manufacturing processes of Plus and Prodigy. Samples were collected from the PreS fractions after incubation (4 h, 37°C, 5% CO 2 ) with the HCMVpp65 peptide pool (MACS GMP PepTivator HCMV <t>pp65,</t> 1 μg/ml per peptide). Flow cytometric analyses were performed to identify total IFN-γ + -releasing CD3 + T-cells (A) and (CD4 + /CD8 + ) T-cell subsets (B,C) in those PreS fractions to compare both restimulation procedures.
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    Jerini Inc pools of 15 amino acid peptides (30) with an overlap of 11 amino acids spanning the entire hcmv proteins pp65 or ie-1
    Comparison of ex vivo restimulation of CMV-specific T-cells by antigenic restimulation by platform . Restimulation frequencies of CMV-specific T-cells were compared between all manufacturing processes of Plus and Prodigy. Samples were collected from the PreS fractions after incubation (4 h, 37°C, 5% CO 2 ) with the HCMVpp65 peptide pool (MACS GMP PepTivator HCMV <t>pp65,</t> 1 μg/ml per peptide). Flow cytometric analyses were performed to identify total IFN-γ + -releasing CD3 + T-cells (A) and (CD4 + /CD8 + ) T-cell subsets (B,C) in those PreS fractions to compare both restimulation procedures.
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    Comparison of ex vivo restimulation of CMV-specific T-cells by antigenic restimulation by platform . Restimulation frequencies of CMV-specific T-cells were compared between all manufacturing processes of Plus and Prodigy. Samples were collected from the PreS fractions after incubation (4 h, 37°C, 5% CO 2 ) with the HCMVpp65 peptide pool (MACS GMP PepTivator HCMV pp65, 1 μg/ml per peptide). Flow cytometric analyses were performed to identify total IFN-γ + -releasing CD3 + T-cells (A) and (CD4 + /CD8 + ) T-cell subsets (B,C) in those PreS fractions to compare both restimulation procedures.

    Journal: Frontiers in Immunology

    Article Title: Comparative Analysis of Clinical-Scale IFN-γ-Positive T-Cell Enrichment Using Partially and Fully Integrated Platforms

    doi: 10.3389/fimmu.2016.00393

    Figure Lengend Snippet: Comparison of ex vivo restimulation of CMV-specific T-cells by antigenic restimulation by platform . Restimulation frequencies of CMV-specific T-cells were compared between all manufacturing processes of Plus and Prodigy. Samples were collected from the PreS fractions after incubation (4 h, 37°C, 5% CO 2 ) with the HCMVpp65 peptide pool (MACS GMP PepTivator HCMV pp65, 1 μg/ml per peptide). Flow cytometric analyses were performed to identify total IFN-γ + -releasing CD3 + T-cells (A) and (CD4 + /CD8 + ) T-cell subsets (B,C) in those PreS fractions to compare both restimulation procedures.

    Article Snippet: Ex vivo restimulation (4 h, 37°C, 5% CO 2 ) with the GMP-grade CMVpp65 peptide pool (MACS ® GMP PepTivator ® HCMV pp65, 1 μg/ml per peptide, Miltenyi Biotec), labeling of WBCs with the CliniMACS CCS ® Catchmatrix Reagent (37°C, 5% CO 2 ), cooling and cooled centrifugation steps (2–6°C), and labeling were carried out manually with the Plus and autonomously within the Prodigy instrument.

    Techniques: Comparison, Ex Vivo, Incubation